Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas: Molecular Genetics; Lung and Breast Carcinomas

Author: M. A. Hayat
Published: May 2004
Publisher: Elsevier Science & Technology
ISBN: 0123339413
Hardcover Book
Number of Pages: 400

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The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.

This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.

Table of Contents
Classification scheme of human cancers
Lung and breast carcinomas
I Introduction
1.1 Comparison of immunohistochemistry, in situ hybridization, fluorescence in situ hybridization, and chromogenic in situ hybridization 1
1.2 Comparison of chromogenic in situ hybridization, fluorescence in situ hybridization, and immunohistochemistry 13
1.3 Target and signal amplification to increase the sensitivity of in situ hybridization 27
II Molecular pathology
2.1 Polymerase chain reaction technology 43
2.2 DNA microarrays technology 49
2.3 Tissue microarrays and their modifications in high-throughput analysis of clinical specimens 57
2.4 Gene expression profiling using laser microdissection in cancer tissues 67
2.5 Differential display of gene expression in human carcinomas 75
2.6 Serial analysis of gene expression in human diseases 85
III Lung carcinoma
3.1 Lung carcinoma : an introduction 99
3.2 Histopathological classification and phenotype of lung tumors 105
3.3 Immunohistochemistry and in situ hybridization of mucin in lung carcinoma 115
3.4 Immunohistochemical expression of MDM2 in lung carcinoma 127
3.5 Immunohistochemical expression of E2F1 and p14[superscript ARF] in lung carcinoma 133
3.6 Role of immunohistochemical expression of beta-catenin in lung carcinoma 141
3.7 Immunohistochemistry of laminin-5 in lung carcinoma 155
3.8 Role of immunohistochemical expression of caveolin-1 in lung carcinoma 163
3.9 Role of thyroid transcription factor-1 in pulmonary adenocarcinoma 169
3.10 Role of global methylation of DNA in lung carcinoma 181
3.11 Immunohistochemical and molecular pathology of angiogenesis in primary lung adenocarcinoma 189
3.12 Immunohistochemistry of human leukocyte antigen expression in lung carcinoma 199
3.13 Immunohistochemistry and in situ hybridization of telomerase in lung carcinoma 205
3.14 Use of fluorescence in situ hybridization in detection of lung cancer cells 213
3.15 Immunohistochemistry of BCL-2 gene expression in lung carcinoma 223
IV Breast carcinoma
4.1 Breast carcinoma : an introduction 231
4.2 Expression of vascular endothelial growth factor receptor-2Flk-1/KDR in breast carcinoma 249
4.3 HER-2/neu amplification and protein overexpression in breast carcinoma : immunohistochemistry and fluorescence in situ hybridization 259
4.4 HER-2/neu amplification detected by fluorescence in situ hybridization in cytological samples from breast cancer 267
4.5 Detection of HER-2 oncogene in human breast carcinoma using chromogenic in situ hybridization 279
4.6 Innunohistochemical evaluation of sentinel lymph nodes in breast carcinoma 289
4.7 CD10 expression in normal breast and breast cancer tissues 299
4.8 Role of immunohistochemical expression of AKT protein in breast carcinoma 307
4.9 Expression of extracellular matrix proteins in breast cancer 321
4.10 Immunohistochemistry of adhesion molecule CEACAM1 expression in breast carcinoma 337
4.11 Role of cadherins in breast cancer 343
4.12 Immunohistochemical expression of erythropoietin and erythropoietin receptor in breast carcinoma 351
4.13 Loss of BRCA1 gene expression in breast carcinoma 361
4.14 Role of immunohistochemical defection of BRCA1 in breast cancer 371
4.15 Fluorescence in situ hybridization of BRCA1 gene in breast carcinoma 385
4.16 Immunohistochemistry of c-myc expression in breast carcinoma 395
4.17 Immunohistochemical localization of neuropilin-1 in human breast carcinoma : a possible molecular marker for diagnosis 409
4.18 Role of the epidermal growth factor receptor in breast carcinoma 415
4.19 Alterations of the cell cycle regulating proteins in invasive breast cancer : correlation with proliferation, apoptosis, and clinical outcome 425
4.20 Immunohistochemistry of estrogen receptor expression in breast carcinoma 439
4.21 Immunofluorescence and immunohistochemical localization of progesterone receptors in breast carcinoma 449
4.22 Immunohistochemical expression of cytosolic thymidine kinase in patients with breast carcinoma 463
4.23 Immunohistochemical detection of melanoma antigen E (MAGE) expression in breast carcinoma 471
4.24 Role of immunohistochemical expression of receptors in male breast carcinoma 477
4.25 Detection of glycoconjugates in breast cancer cell lines : confocal fluorescence 487
4.26 Role of ETV6-NTR23 gene fusion in breast carcinoma 493
4.27 Role of CA6 protein expression in breast carcinoma 505
4.28 Immunohistochemistry of effusions 513
4.29 Immunohistochemistry of needle cytopunctures of breast carcinomas 523

Click to compare book prices for Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas: Molecular Genetics; Lung and Breast Carcinomas Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas: Molecular Genetics; Lung and Breast Carcinomas

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