Immunology of Infection, Vol. 32

Author: Stefan H.E. Kaufmann
Published: December 2002
Publisher: Academic Press, Incorporated
ISBN: 012402033X
Paperback Book
Number of Pages: 806

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Immunology of Infection, 2nd Edition, edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist interested in analysing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology.

This new edition of Immunology of Infection provides ready-to-use "recipes", and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the successful first edition.

Methods in Microbiologyis the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.

Key Features:

* Includes techniques for genome-wide expression profiling of both the pathogen and host and of the host response to infection
* Cytometric analysis of cytokine secretion by immune cells
* Describes tetramer technology for the quantitative analysis of antigen specific T cell responses
* analysis of host cells and pathogens involved in the host-microbe interplay
* Covers techniques useful for the analysis of human and murine systems
* Includes techniques for the prediction and determination of MHC ligands and T cell epitopes
* Covers the fundamentals and practice of DNA vaccines
* Describes methods for the isolation and propagation of human dendritic cells

Table of Contents
Contributors
Preface to the First Edition
Preface to the Second Edition
Introduction: The immune response to infectious agents 1
I General Methods
1 Phenotyping and separation of leukocyte populations based on affinity labelling 23
2 Cytometric cytokine secretion assay: detection and isolation of cytokine-secreting T cells 59
3 Measurement of cellular proliferation 77
4 Use of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope-driven vaccine design 99
5 The use of tetramers in the quantitative analysis of T-cell responses 125
6 Using microarrays for studying the host transcriptional response to microbial infection 157
7 Genome-wide expression profiling of intracellular bacteria: the interaction of Mycobacterium tuberculosis with macrophages 169
II Murine Models
1 Management of immunocompromised and infected animals 183
2 In vitro analysis
2.1 Isolation and preparation of lymphocytes from infected animals for in vitro analysis 233
2.2 Isolation and development of murine T-lymphocyte hybridomas and clonal cell lines 249
2.3 Killer cell assays 281
2.4 Quantitation of T-cell cytokine responses by ELISA, ELISPOT, flow cytometry and reverse transcriptase-PCR methods 301
2.5 Isolation of and measuring the function of professional phagocytes: murine macrophages 331
3 In vivo analysis
3.1 The immune response in mice challenged with bacterial infections 359
3.2 Measuring immune responses in vivo 403
4 Specific Models
4.1 Murine models of tuberculosis 433
4.2 The leishmaniasis model 463
4.3 Animal models: murine cytomegalovirus 493
4.4 DNA vaccines: fundamentals and practice 527
4.5 Preparation and use of adjuvants 551
III Human Systems
1 Isolation and propagation of human dendritic cells 591
2 Isolation of T cells and establishment of T-cell lines and clones 621
3 Growth transformation of human T cells 657
4 Generation and characterization of human killer cells 693
5 Measuring human cytokine responses 707
6 Measuring immune responses in situ: immunofluorescent and immunoenzymatic techniques 751
7 Isolation, characterization and cultivation of human monocytes and macrophages 767
Index 787

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