Analytical Use of Fluorescent Probes in Oncology

Author: Elli Kohen
Published: March 1997
Publisher: Kluwer Academic Publishers
ISBN: 030645369X
Hardcover Book
Number of Pages: 448

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Microscopy w/transduction, laser scanning confocal fluorescence microscopy of cell function. Forty-seven proceedings papers from the October 1995 workshop highlighting the significance of fluorescence work for the understanding of cell and tissue physiology, physiopathology and pharmacology, particularly in terms of the analytical use of fluorescent probes in oncology. The research presentations describe "in vivo" and "in situ" studies using fluorescence and related optical methods to explore tissues, cells, and organelles photon effects previously understood only in solution photochemistry. Among the highlighted papers were those dealing with "in vivo" spectroscopy and imaging, the current study of signal transduction by using fluorescence resonance energy transfer, and three-dimensional imaging of calcium-loaded myocytes. .

Table of Contents
1 Overview: Workshop on Analytical Use of Fluorescent Probes 1
2 Detection of Breast Tumors by Absorption and Fluorescence of Photon Diffusive Waves 25
3 Fluorescence-Lifetime Based Sensing in Tissues and Other Random Media with Measurements of Photon Migration 31
4 Spectroscopy and Tomography of Tissues in the Frequency-Domain 41
5 Fret Microscopy in Cellular Signal Transduction 53
6 Photoacoustic Spectroscopy Applied to Biological Materials 59
7 Recent Developments in Fluorescence Spectroscopy: Long-Lived Metal-Ligand Probes, Multi-Photon Excitation, and Control of the Excited State 65
8 Photonic Pathology: Fluorescence and Raman Spectroscopy for Tissue Diagnosis and Characterization 81
9 In Vivo Application PD-Porphine for Measurement of Oxygen Concentrations in the Gut 91
10 Laser Scanning Confocal Fluorescence Microscopy of Cell Function 97
11 Sub-Cellular Localization of Second Generation PDT Photosensitizers Studied by Microspectrofluorometry 113
12 Photofrin Uptake as a Function of Tumor Size and the Detection of Microscopic Nests of Tumor Cells by Photofrin Fluorescence: Mechanistic Implications for PDT 123
13 The Detection of Apoptotic Cells and Their Quantification by Flow Cytometry 129
14 Adhesion Stabilization of Blood Borne Cancer Cells in the Microcirculation 137
15 Probing of Connexin Channels: Evidence for Involvement of Junctional Coupling in Pancreatic Secretion 149
16 The Analysis of Drug-Induced Cell Cycle Perturbations by Flow Cytometry 157
17 The Effects of Tumor Heterogeneity on the Flow Cytometric Analysis of Clinical Specimens 173
18 Videomicrofluorometry of Progesterone Receptors and Their Genes in Breast Cancer Cells 181
19 Immunophotodetection of Cancer by Antibody-Indocyanin Conjugates: Experimental and Preliminary Clinical Results 189
20 Three-Dimensional Optical Functional Imaging of Tissue with Two-Photon Excitation Laser Scanning Microscopy 205
21 Estrogen Receptor Imaging Using Intrinsically Fluorescent Ligands 213
22 Videomicrofluorometry and Intracellular Ionic Concentration in Single Living Cells: From Toy to Tool 221
23 Multiwavelength Microfluorometry to Investigate Cytotoxic Processes on Sensitive and Resistant Human Lymphoblastoid CCRF-Cem Lines 231
24 Laser-Induced Auto Fluorescence of Normal and Tumor Bladder Cells and Tissues: In Vitro and in Vivo Studies 241
25 Fluorescent Imaging of Membrane Potential Components 249
26 Probing of Microtubule Structures with Monoclonal Antibodies and Immunofluorescence Microscopy 257
27 Immunofluorescence Detection of Cytoskeletal Structures 265
28 Cell Expression System in V79 Cells Transfected with Cytochrome P450 and Its Performance in Metabolism Studies 273
29 Direct Transfer of Control and Multidrug Resistance 283
30 Instrumentation Design for Study of Metabolic Control in Living Cells 293
31 Use of Fluorescent Derivatives for Labeling Cells: Diagnosing, Preventing, and Correcting Genetic Diseases: Photosynthesizing and Killing of Tumors, Parasites, and Viruses 299
32 Ca[superscript 2+] Signaling and Cytoskeleton Reorganization in Endothelial Cells Stimulated by Bradykinin 309
33 Integration of Optical Techniques in Scanning Probe Microscopes: The Scanning Near-Field Optical Microscope (SNOM) 317
34 Perfusion, Temperature, and PO[subscript 2] Control in a Leiden Culture Bath for Single Cell Measurements and Manipulations 325
35 Immunofluorescence Study of Intermediate Filament Protein Expression under Normal and Pathological Conditions 331
36 The Use of Fluorescent Probes in Characterizing Drug Resistance in Cultured Cells 339
37 A High Resolution Multimode Digital Imaging System for Fluorescence Studies of Mitosis 349
38 Use of Photodiode Array Fluorescence Spectroscopy in Biochemistry, Pharmacology, and Oncology 357
39 Hydrophobic Photosensitizers Delivery to Tumor Tissues by an Amphiphilic Peptide: In Vitro and in Vivo Fluorimetric Studies 363
40 Merocyanine 540 - A Fluorescent Dye and a Biological Probe 371
41 High Pressure Infrared Spectroscopy in Biological and Biomedical Studies 383
42 Fluorescence Spectroscopy for Diagnosis of Cancerous Tissues 393
43 Solvent-Dependent Excited State Properties of Psoralens: A Model to Their Antiproliferative Action 405
44 Fluorescent Probes in Breast Tumor 411
45 Use of Fluorescence in Situ Hybridization for Mapping and Ordering YAC Clones from Chromosomal Regions with Suspected Tumor-Suppressor Activity 419
46 Photochemical and Photophysical Studies of an Anthocyanin: Fast Excited-State Proton Transfer from 4-Methyl 7-Hydroxyflavylium Chloride to Water 423
47 Selectivity and Localization of Anthracyclines in Cardiac-Muscle and Non-Muscle Cells Relevance to Multidrug Resistance 425
Index 427

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