Flow Cytometry Principles for Clinical Laboratory Practice: Quality Assurance for Quantitative Immunophenotyping

Author: Marilyn A. Owens
Published: October 1994
Publisher: Wiley, John & Sons, Incorporated
ISBN: 0471021768
Paperback Book
Number of Pages: 228

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Provides authoritative coverage of accepted methods, quality controls and standards for the cytometric analysis of clinical samples with particular relevance to CD4+ lymphocytes. CD4+ lymphocyte determination by immunophenotyping is the standard method for evaluating the status of HIV infection and the single most frequent application of flow cytometry in a diagnostic setting. This is a new book written by two flow cytometry experts as a guide to quality assurance necessary for quantitative immunophenotyping by flow cytometry using clinical cases of CD4+ T cell enumeration and CD34+ stem cell quantitation in HIV infection and peripheral blood stem cell transplantation. The purpose is to describe the theory and practice of quality assurance in quantitative flow cytometric immunophenotyping in a ready-reference format for diagnostic laboratories for daily use. With accurate instrument maintenance and setup an absolute requirement for clinical sample analysis, this book does well to inform the laboratory personnel of the necessary procedures to ensure accurate results and above all, to comply with regulations applied to clinical laboratories using flow cytometers. Primarily intended for laboratory directors and laboratory technologists whose duties may include quantitative immunophenotyping by flow cytometry, this book may be recommended as a quick reference for other personnel desiring to troubleshoot an instrument that is performing suboptimally. The authors are experts in the field of flow cytometric analysis of peripheral blood who write very clearly at the intended level of their audience. The book is well referenced and each chapter concludes with a series of review questions that are useful in reviewing current laboratory procedures as well as self-assessment for laboratory personnel. The illustrations are bold histograms with individual legends as well as textual references. This is a laboratory guide to quality assurance in quantitative immunophenotyping of peripheral blood cells by flow cytometric analysis. It is concise, with detailed approaches to twofundamental clinical problems of quantitative CD4+ T cell counts in HIV disease and CD34+ peripheral stem cell counts in bone marrow transplantation. It is suggested for laboratory technologists, laboratory directors in larger hospitals, and medical school libraries. Owens, Marilyn A. (Cytometry Associates, Inc., CA); Loken, Michael R. (Cytometry Associates, Inc., WA)

Table of Contents
Acknowledgments
I Assumptions in Flow Cytometry 1
II Components of Flow Cytometric Immunophenotyping 17
III Quality Control of Flow Cytometer and Reagents 45
IV Quantitative Immunophenotyping in HIV Infection 73
V Peripheral Blood Stem Cell Quantitation 111
VI Variability in Immunophenotyping 129
VII Safety and Specimen Acquisition, Handling, and Processing 155
VIII Analysis of FCI Results and Troubleshooting 175
IX New Methods for Cell Enumeration
Index 217

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