Cardiovascular Physiology in the Genetically Engineered Mouse

Author: Brian D. Hoit
Published: January 2002
Publisher: Kluwer Academic Publishers
ISBN: 079237536X
Hardcover Book
Number of Pages: 424

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Cardiovascular Physiology in the Genetically Engineered Mouse serves as a practical, "how-to guide" for the investigator interested in the functional methods used to characterize the murine cardiovascular phenotype. It is assumed that neither the cardiovascular physiologist nor the molecular biologist have a significant familiarity with each other's field; in this sense the content is truly introductory, and therefore valuable for both the scientist/physician and trainee. A second objective is to help decipher the literature critically and foster an understanding of the advantages and limitations of each method. Molecular biology, and more specifically, transgenesis and gene targeting, have progressed at a rapid pace; this work aims to provide a framework for the large amount of information in this luxuriating, and often bewildering field. The multidisciplinary nature of the book will facilitate the newly-formed marriage between physiologists and molecular biologists and will stimulate and direct young investigators toward the field.

Table of Contents:

Preface
1 The Hierarchial Approach to Analysis of Cardiovascular Function in Mice: An Overview 1
2 Targeted Ablation of Murine Cardiac Genes 17
3 Transgenic Models of Cardiovascular Physiology and Pathophysiology 37
4 Isolated Myocyte Mechanics and Calcium Transients 55
5 The Isolated Isovolumic (Langendorff) Heart Preparation 69
6 The Isolated Work-Performing Mouse Heart Preparation. Comparison and Quantification of Cardiac Performance in Transgenic and Wild-Type Mice 77
7 Echocardiographic Assessment of the Mouse Heart and Aorta 97
8 Invasive Hemodynamic Studies in Open and Closed Chest Mice 111
9 Cardiovascular Physiology in Mice: Conscious Measurements and Effects of Anesthesia 125
10 Single Cell Patch-Clamp Analysis of Mouse Myocytes 145
11 In Vivo Electrophysiologic Studies in Mice 161
12 [superscript 31]p NMR Spectroscopy of the Mouse Heart 183
13 Models of Cardiac Disease in the Mouse 193
14 Exercise and Oxygen Consumption in the Mouse 213
15 Mouse Smooth Muscle Studies Using Gene-Altered Mouse Models: A Users' Guide 247
16 Analysis of Murine Embryonic Structural and Functional Phenotype 259
Index 281

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