Immunological and Molecular Diagnosis of Infectious Disease

Author: Daniel Amsterdam
Published: March 1997
Publisher: Marcel Dekker
ISBN: 0824700929
Hardcover Book
Number of Pages: 295

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Based on the XIIIth International Convocation on Immunology held recently at the State University of New York at Buffalo, this timely resource highlights the latest developments in the clinical diagnosis of several major emerging and reemerging infectious diseases. Addressing both immunological and molecular methodologies of diagnosis as well as the clinical aspects of diseases, Immunological and Molecular Diagnosis of Infectious Disease discusses DNA and RNA amplification methods...explains ELISA approaches...introduces rapid diagnosis techniques, biosensors, and flow cytometry...examines bacterial and parasitic infections, including M. tuberculosis, Borrelia burgdorferi, Streptococcus pyogenes, chlamydia, and schistosomes...describes viral infections such as hantaviruses, hepatitis C, cytomegalovirus, herpes viruses, and HIV...treats syndromes with infectious origins, including prions, chronic fatigue syndrome, and superantigens...and much more. Written by world-renowned experts, Immunological and Molecular Diagnosis of Infectious Disease is an excellent resource for immunologists, infectious disease specialists, microbiologists, virologists, bacteriologists, parasitologists, clinical chemists and biochemists, epidemiologists, pharmacologists, and graduate-level students in these disciplines.

Table of Contents:

Preface iii
Contributors ix
I. Immunological and Molecular Approaches
1. DNA Amplification 3
2. Branched DNA for Quantification of Viral Load 9
3. NASBA Technology: Isothermal RNA Amplification in Qualitative and Quantitative Diagnostics 15
4. Concerted Use of Immunologic and Ultrastructural Analyses in Diagnostic Medicine: Immunoelectron Microscopy and Correlative Microscopy 29
5. Adsorption-Induced Antigenic Changes and Their Significance in ELISA and Immunological Disorders 39
6. Rapid Diagnosis of Periodontal Infections: Findings in AIDS Patients 55
7. Uses of Biosensors in the Study of Viral Antigens 67
8. Identification and Function of Host Defense Cells by Flow Cytometry 83
9. Application of Flow Cytometry in Transplantation Medicine 91
II. Bacterial and Parasitic Infections
10. Critical Assessment of Gene Amplification Approaches on the Diagnosis of Tuberculosis 105
11. Borrelia burgdorferi Infection: Clinical Diagnostic Techniques 117
12. The Toxins of Group A Streptococcus, the Flesh Eating Bacteria 129
13. Diagnosis of Chlamydial Infection in the Pediatric Population 151
14. DFA, EIA, PCR, LCR, and Other Technologies: What Tests Should be Used for Diagnosis of Chlamydia Infections? 157
15. Molecular Diagnosis of Helicobacter pylori 163
16. Immunodiagnosis of Schistosomiasis 175
III. Viral Infections
17. The Evolution of Hantaviruses 191
18. Laboratory Diagnosis of Hepatitis C 199
19. Polymerase Chain Reaction Assays for the Detection of Cytomegalovirus in Organ and Bone Marrow Transplant Recipients 209
20. HTLV-Associated Diseases: Human Retroviral Infection and Cutaneous T-Cell Lymphomas 231
21. Management of Cytomegalovirus Infection in Solid-Organ Transplant Recipients 243
IV. Syndromes of Various Infectious Origins
22. Immunodiagnosis of Prion Disease 259
23. Chronic Fatigue Syndrome 269
24. Superantigens: Their Role in Infectious Diseases 275
25. Searching for Superantigens 283
Author Index 291
Subject Index 293

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