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Preface
Contributors
1 Characterization and Enrichment of Human Hematopoietic Stem Cells 1
2 Isolation and Characterization of Hematopoietic Stem Cells 27
3 Embryonal Stem Cells and Hematopoiesis: Current Perspectives 57
4 Developmental Potential of Fetal Hematopoietic Stem Cells 69
5 Phenotypic and Functional Analysis of Hematopoietic Stem Cells in Mouse and Human 85
6 Hemopoietic Stem-Cell Homing: Potential Modulation by Cytokines 125
7 The Maintenance of Hemopoietic Stem Cells in Human Long-Term Marrow Culture 151
8 Ex Vivo Expansion of Neutrophil Precursors from Blood CD34+ Cells: Applications for the Treatment of Neutropenia 171
9 Rationale and Methods for Retroviral-Mediated Gene Transfer into Peripheral Blood Hematopoietic Progenitors 185
10 Therapy with Genetically Modified Hematopoietic Stem Cells 195
11 Genetic Modification of Hematopoietic Stem Cells for Therapy of Solid Tumors and Hematopoietic Neoplasms 219
12 Retroviral Gene Transfer into Hematopoietic Stem Cells: The Human MDR Gene as a Model System 229
13 Therapeutic Applications of HSC: Retroviral-Mediated Gene Transfer and Gene Therapy 243
14 Viral Vector Systems for Bone Marrow Gene Therapy 275
15 Human Umbilical Cord Blood Transplantation: The Immunology, Expansion, and Therapeutic Applications of Hematopoietic Stem and Progenitor Cells 297
16 Umbilical Cord Blood Cells: Immunological Analysis in View of Clinical Applications 319
17 Use of Human Umbilical Cord Blood for Stem-Cell Transplantation (HLA-Matched, Unmatched: Clinical, Ethical, and Legal Aspects) 333
18 Hematopoietic Progenitor/Stem Cells in Cord Blood, Neonates, Infants, and Children 349
19 In Utero Stem-Cell Therapy, Using Fetal Cells Transplanted into Fetal Recipients 369
20 Peripheral Blood Stem Cells: Possibilities and Limitations 391
21 Mobilization of Peripheral Blood Stem Cells: Are All Regimens Created Equal? 403
22 Autologous Blood Stem-Cell Versus Bone Marrow Transplantation: Comparison of Cost-Effectiveness and of Clinical Benefits 421
23 Mobilization of Stem Cells with Cytokines 435
24 Cytokine Priming of Bone Marrow and Blood Progenitors Before Harvest for Autologous Stem-Cell Transplantation 455
25 Mobilization of Peripheral Blood Stem Cells with Cytokines 467
26 Ex Vivo Expansion of Human Hemopoietic Progenitors with Cytokines 491
27 Selection and Transplantation of Autologous Hematopoietic Progenitor Cells 519
28 Transplantation with Hematopoietic Stem Cells: Dose-Intensive Therapy in Breast Cancer 535
29 Autologous Hematopoietic Stem-Cell Transplantation in Malignant Lymphomas 557
30 Hematopoietic Stem-Cell Transplantation in Multiple Myeloma 571
31 High-Dose Chemotherapy with Hematopoietic Stem-Cell Support in the Treatment of Epithelial and Mesenchymal Neoplasms: Lung Cancer, Ovarian Cancer, Germ-Cell Malignancies, and Soft Tissue Sarcoma 589
32 Application of Recombinant Granulocyte Colony-Stimulating Factor in Peripheral Blood Stem-Cell Transplantation: A Pediatric Experience 611
33 Bone Marrow Transplantation for Severe Aplastic Anemia 631
Index 643