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Methods in Renal Toxicology presents a vast array of methods for the study of renal cellular and tubular structure, function, and biochemistry under physiological, toxicological, and pathological conditions. It provides detailed descriptions in easy-to-understand language of methods designed to enhance your research efforts.

Methods in Renal Toxicology puts you on the cutting edge with valuable chapters detailing molecular methods and transgene and gene targeting - the most recent approaches to the study of renal toxicology. Overall, the book's topics range from non-invasive assessments of renal function in the whole animal and clinical settings to cellular and molecular approaches. Specifically, the book delves into measurements of clearance and urinary markers, histopathology, and methods to assess renal carcinogenesis, mutagenesis, oxidative stress, mitochondria injury, cellular repair, and drug metabolism and transport. A variety of in vitro methods are also described, including the isolated perfused kidney, micropuncture, microperfusion, microdissection, renal slices, isolated perfused tubules, suspensions of tubules and isolated cells, and primary cell cultures and cell lines.

Methods in Renal Toxicology is a must-have resource for all renal investigators.

Nowhere else can you find concise descriptions of traditional and up-to-the-minute renal toxicology methods in such a practical, well-written single-volume guide.

Table of Contents:

1 Clearance Methods in Renal Toxicology 1
2 Urinary Markers 19
3 Histopathologic Methods in Renal Toxicology 35
4 The Isolated Perfused Kidney 59
5 Micropuncture and Microperfusion Techniques 79
6 Microdissection and Microanalysis of Specific Nephron Segments from Mammalian Kidney 97
7 Preparation and Use of Precision-Cut Renal Cortical Slices in Renal Toxicology 109
8 In Vitro Perfusion of Isolated Nephron Segments: A Method for Renal Toxicology 123
9 Suspensions of Rabbit Renal Proximal Tubules 147
10 Characterization and Use of Rabbit Renal Proximal Tubular Cells in Primary Culture for Toxicology Research 163
11 Use of Freshly Isolated and Primary Cultures of Proximal Tubular and Distal Tubular Cells from Rat Kidney 189
12 Immortalized Cell Lines of Renal Cells 217
13 Methods in Renal Carcinogenesis and Mutagenesis 237
14 Measurement of Reactive Oxygen Species, Oxidative Damage, and Antioxidant Systems in the Kidney 255
15 Mechanisms of Repair After Renal Injury 283
16 Mitochondrial Toxicity in Renal Injury 299
17 Biotransformation and Membrane Transport in Nephrotoxicity 331
18 Methods to Evaluate Renal Cytochrome P450 as a Bioactivation System for Nephrotoxicants 353
19 Molecular Methods in Renal Toxicology 377
20 Transgenic Animals 413
Index 427