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Pt. I Immuno-Gold Electron Microscopy for Virus Diagnosis
Ch. 1 Immune Complexing 3
Ch. 2 Solid Phase Immune Electron Microscopy of Virus Preparations 25
Ch. 3 Gold Labeling in Negative Stain Immune Electron Microscopy 71
Ch. 4 The Grid-Cell-Culture Technique 89
Ch. 5 Use of Microspheres for Immune Scanning Electron Microscopy 113
Pt. II Immuno-Gold Electron Microscopy in Virus Research
Ch. 6 Colloidal Gold Probe: An Overview of Its Application in Viral Cytochemistry 137
Ch. 7 Detection of Viral and Cellular Structures by Post-Embedding Immunocytochemistry 177
Ch. 8 Multiple Labeling of Thin Sections 231
Ch. 9 Immuno-Gold Replica Technique for Study of Virus-Infected Cells 257
Ch. 10 Detection and Analysis of Viral Proteins by Immuno-Gold Labeling 289
Ch. 11 Cryo-Sectioning 309
Ch. 12 Ultrasmall Gold Probes and Cryo-Ultramicrotomy 327
Ch. 13 Freeze Substitution in Virus Research: A Preview 349
Ch. 14 Ultrastructural In Situ Hybridization Using Immuno-Gold 377
Ch. 15 Immunoscanning Electron Microscopy: The Application of Colloidal Gold and Silver Enhancement 411
Index 467