Electrochemotherapy, Electrogenetherapy and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells, Vol. 37

Author: Mark J. Jaroszeski
Published: February 2000
Publisher: Humana Press
ISBN: 0896036065
Hardcover Book
Number of Pages: 497

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The use of electricity to mediate the delivery of molecules to cells in vivo is a growing application of our greater ability to deliver drugs, DNA, antibodies and proteins into the cells of living organisms. This volume provides the techniques and methods for in vivo electroporation covering electrochemotherapy, electrogenetherapy, and transdermal delivery of pharmacological agents by electroporation. The first section of this state-of-the-art manual reviews the critical aspects of electroporation, chemotherapeutic agent delivery, gene delivery and transdermal transport providing information about the history, current state, and future implications of the work that has been accomplished using electric fields to deliver molecules to cells in vivo. The second section focuses on protocols for the delivery of molecules into cells and through the skin, and are divided into sections, based on their relevance to chemotherapeutic agent delivery, gene delivery, and transdermal delivery. Based on the highly successful clinical trials using electrochemotherapy, the promising gene therapy results in animals, along with the encouraging progress being made with transdermal delivery, this book will help lead the way for this field's expansion into the clinical domain in the near future.

Table of Contents:

Preface
Contributors
Reviews
1 Principles of Membrane Electroporation and Transport of Macromolecules 1
2 Instrumentation and Electrodes for In Vivo Electroporation 37
3 Numerical Modeling for In Vivo Electroporation 63
4 In Vitro Delivery of Drugs and Other Molecules to Cells 83
5 The Basis of Electrochemotherapy 99
6 Electrochemotherapy: Animal Model Work Review 119
7 Clinical Trials for Solid Tumors Using Electrochemotherapy 137
8 In Vitro and Ex Vivo Gene Delivery to Cells by Electroporation 157
9 Delivery of Genes In Vivo Using Pulsed Electric Fields 173
10 Mechanism of Transdermal Drug Delivery by Electroporation 187
11 Mechanistic Studies of Skin Electroporation Using Biophysical Methods 213
Electrochemotherapy Protocols
12 Treatment of Murine Transplanted Subcutaneous Tumors Using Systemic Drug Administration 247
13 Electrochemotherapy of Murine Melanoma Using Intratumor Drug Administration 253
14 Treatment of a Tumor Model with ECT Using 4+4 Electrode Configuration 259
15 Treatment of Multiple Spontaneous Breast Tumors in Mice Using Electrochemotherapy 265
16 Electroporation of Muscle Tissue In Vivo 271
17 Treatment of Human Pancreatic Tumors Xenografted in Nude Mice by Chemotherapy Combined with Pulsed Electric Fields 277
18 Distribution of Bleomycin in a Rat Model 285
19 Treatment of Rat Bladder Cancer with Electrochemotherapy In Vivo 293
20 Electrochemotherapy for the Treatment of Soft Tissue Sarcoma in a Rat Model 299
21 Treatment of Spontaneous Soft Tissue Sarcomas in Cat 305
22 Treatment of Rat Glioma with Electrochemotherapy 313
23 Treatment of Liver Malignancies with Electrochemotherapy in a Rat Model 319
24 Treatment of Liver Tumors in Rabbit 327
Electrogenetherapy Protocols
25 Electrically Mediated Reporter Gene Transfer into Normal Rat Liver Tissue 333
26 Reporter/Functional Gene Transfer in Rat Brain 339
27 In Vivo Gene Electroporation in the Mouse Testis 349
28 Ex Vivo Stromal Cell Electroporation of Factor IX cDNA for Treatment of Hemophilia B 359
29 In Ovo Gene Electroporation into Early Chicken Embryos 369
Transdermal Delivery Protocols
30 Electrical Impedance Spectroscopy for Rapid and Noninvasive Analysis of Skin Electroporation 377
31 An In Vitro System for Measuring the Transdermal Voltage and Molecular Flux Across the Skin in Real Time 407
32 Using Surface Electrodes to Monitor the Electric-Pulse-Induced Permeabilization of Porcine Skin 437
33 Transdermal Delivery Using Surface Electrodes in Porcine Skin 447
34 Transdermal Drug Delivery by Skin Electroporation in the Rat 457
35 In Vivo Skin-Targeted Gene Delivery by Pulsed Electric Fields 473
Index 489

Click to compare book prices for Electrochemotherapy, Electrogenetherapy and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells, Vol. 37 Electrochemotherapy, Electrogenetherapy and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells, Vol. 37

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