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To date, there has been no collection of these assays in a single volume that succinctly described the inherent value and limitation of each experimental approach. This volume enables both basic scientists and clinical researchers to isolate and functionally characterize purified stem cells from various sources, to genetically modify stem cells using viral gene delivery systems, and to describe various molecular analyses that can be performed iwth a small number of cells.

Forty-one international specialists contribute 20 chapters to this volume, providing a variety of key laboratory methods for investigating hematopoietic stem cells (HSC). The text includes approaches using mouse and human tissues of both embryonic and adult origin, methods for the purification and genetic modification of stem cells, stem cell expansion protocols, and in vitro and in vivo assays, with techniques for studying a variety of stem cell topics, including blood formation, blood-based disease, and blood cancers such as leukemia. Accessible to both experienced investigators and newcomers to the field of HSC research.