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Table of Contents:

Dedication v
Foreword vii
Foreword ix
Preface xi
Contributors xvii
Contents for Volume 1 xxi
Part I. Viral-Specific Immunological and Other Host Responses
1 Studying Host Immune Responses Against Duck Hepatitis B Virus Infection 3
2 Measurement of Cell-Mediated Immune Response in Woodchucks 27
3 Study of Liver-Specific Expression of Cytokines During Woodchuck Hepatitis Virus Infection 37
4 Induction of Anti-Hepatitis B Virus Immune Responses Through DNA Immunization 43
5 Monitoring Gene Expression Using DNA Microarrays During Hepatitis B Virus Infection 55
6 Determination of Hepatitis B Virus-Specific CD8[superscript +] T-Cell Activity in the Liver 65
7 Determining the Precursor Frequency of HBV Nucleocapsid Antigen-Specific T Cells 85
8 Detection and Characterization of Virus-Specific CD8[superscript +] T Cells Using the Tetramer Approach 89
9 In Vitro Analysis of Hepatitis B Virus Specific CD4[superscript +] T Cells 97
10 Induction of Humoral and Cellular Immune Responses to Hepatitis Delta Virus Through DNA Immunization in BALB/c Mice 111
Part II. In Vitro and In Vivo Models
11 Infection of Primary Chimpanzee Hepatocytes with Recombinant Hepatitis D Virus Particles: A Surrogate Model for Hepatitis B Virus 131
12 Study of the Endocytosis and Intracellular Localization of Subviral Particles of Hepatitis B Virus in Primary Hepatocytes 143
13 The Tupaia Model for the Study of Hepatitis B Virus: Direct Infection and HBV Genome Transduction of Primary Tupaia Hepatocytes 153
14 Delivery of Hepatitis B Virus Therapeutic Agents Using Asialoglycoprotein Receptor-Based Liver-Specific Targeting 163
15 Woodchuck Hepatitis Virus Hepatocyte Culture Models 175
16 Duck Hepatitis B Virus Primary Hepatocyte Culture Model 189
17 Enhancement of Infection of HepG2 Cells in Culture by Predigestion of Hepadnavirus with V8 Protease 199
18 Construction of Recombinant Adenoviruses that Produce Infectious Hepatitis B Virus 209
19 Baculovirus-Mediated Gene Transfer for the Study of Hepatitis B Virus 219
20 Transgenic Hepatitis B Virus Mouse Model in the Study of Chemotherapy 239
21 Transplantation of Human Hepatocytes in Immunodeficient UPA Mice: A Model for the Study of Hepatitis B Virus 253
22 Duck Hepatitis B Virus Model in the Study of Hepatitis B Virus 261
23 Hepatitis B Virus Transgenic Severe Combined Immunodeficient Mouse Model of Acute and Chronic Liver Disease 269
24 The Chimpanzee Model: Contributions and Considerations for Studies of Hepatitis B Virus 289
25 Hepatitis B in Liver Transplant Recipients as a Special Model of Antiviral Drug Development 319
26 Hepatitis Delta Virus Transfection for the Mouse In Vivo Model 343
27 Hepatitis Delta Virus RNA Transfection for the Cell Culture Model 351
Part III. Antiviral Testing and Clinical Studies
28 Analysis of Hepatitis B Virus Dynamics and Its Impact on Antiviral Development 361
29 Genotyping Anti-Hepatitis B Virus Drug Resistance 379
30 A Parsimonious Method for Screening Drug Combinations for Antihepadnaviral Activity Using a Parametric Dose-Response Surface Approach 387
31 Hepatitis B Virus: Where Are We and Where Are We Going? 415
32 Designing Studies to Evaluate Anti-Hepatitis B Virus Therapies: From the Perspective of Studies for the Registration of Pharmaceutical Products 445
33 Specific Considerations in the Design of Hepatitis B Virus Clinical Studies in the Far East 457
34 Studying the Treatment of Chronic Hepatitis B Viral Infection in Special Populations 465
35 Designing Clinical Development Programs for Anti-Hepatitis B Virus Drugs 499
36 Testing Antivirals Against Hepatitis Delta Virus: Farnesyl Transferase Inhibitors 539
Index 555