Developmental Hematopolesis: Methods and Protocols

Author: Margaret H. Baron
Published: January 2004
Publisher: Humana Press
ISBN: 1588292967
Hardcover Book
Number of Pages: 350

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Table of Contents
Preface v
Contributors xi
Part I Genetic Approaches
1 Gene-Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mouse 3
2 Inducible Transgene Expression in Mouse ES Cells 23
3 Quantitative Trait Analysis in the Investigation of Function and Aging in Hematopoietic Stem Cells 47
Part II Analysis of Cell Fate Outcomes in Transplantation Models
4 Flow Cytometric Analysis of Hematopoietic Development 65
5 Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells 81
6 Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Mice 95
Part III Animal Models
Drosophila
7 Hemocyte Development During Drosophila Embryogenesis 109
Xenopus
8 Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos 123
9 Fate Mapping Hematopoietic Lineages in the Xenopus Embryo 137
10 Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J Strain) 149
11 Use of Flow Cytometry and Combined DNA/Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryo 159
Zebrafish
12 Analysis of Hematopoietic Development in the Zebrafish 171
13 Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos 199
Chick
14 In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo 215
Mouse
15 Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Development 231
16 Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo 257
17 Hematopoietic Stem Cell Development During Mouse Embryogenesis 273
18 Analysis of Hematopoietic Progenitors in the Mouse Embryo 289
19 In Vivo and In Vitro Assays of Thymic Organogenesis 303
20 Retroviral Transduction in Fetal Thymic Organ Culture 311
21 Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors 323
22 A New Clonal Assay System for Lymphoid and Myeloid Lineages 345
23 Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culture 359
24 Analysis of the Vascular Potential of Hematopoietic Stem Cells 369
25 Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture 381
26 Fluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic Differentiation 395
Human
27 Analysis of Hematopoietic Development During Human Embryonic Ontogenesis 413
28 Hematopoietic Development of Human Embryonic Stem Cells in Culture 425
Part IV Bioinformatics and Functional Genomics
29 A Functional Genomics Approach to Hematopoietic Stem Cell Regulation 439
Index 453

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